The Postdoc Research Projects

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Postdoc Research Project 9

Regulation and interactions of pollen-specific Hsf and chaperone networks

Project description

The control of the cellular heat stress response is linked to the activity of Hsfs and chaperones acting as regulators of Hsfs, and interactions within and between the two families play a key role for adaptation of heat stress gene expression. Differences in transcript and protein levels of Hsfs and Hsps in developing pollen of heat-sensitive and heat-tolerant tomato cultivars have been reported. The project will focus on the functional analyses of the pollen-specific Hsf and Hsp networks. The objectives are:

1) to describe the regulatory cascades involved in the developmental control of Hsf and Hsp expression in pollen,
2) to verify the specific function of individual factors and the role of their interplay in pollen heat stress response, and
3) to identify factor-specific differences mirroring the temperature limits in pollen thermotolerance.

The project involves biochemical, cell biological and physiological approaches. Thereby, it is expected that the postdoc will bring expertise into the project, but he will also find optimal conditions to be trained in the complementary techniques.

Where

The group of Klaus-Dieter Scharf, Institute for Molecular Biosciences, Molecular Cell Biology of Plants, Goethe University Frankfurt am Main, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Campus Riedberg, Germany, scharf@bio.uni-frankfurt.de, +49 (0)69 79829 283.

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Postdoc Research Project 10

Transcriptomes and epigenomes of germplasm and breeding lines contrasting in their response to heat stress

Project description

Plant responses to environmental stress involve concerted re-regulation of transcription of thousands of genes. Reports on the contribution of small RNAs and Natural Antisense Transcripts (NATs) to stress tolerance are only emerging and the epigenetic control of transcription and its regulation has been described for only a few loci in model plants. Consequently, the systems biology of stress tolerance requiring such knowledge is still in its infancy. The project includes the high throughput analysis of the transcriptome and epigenetic modifications in different cell types based on advanced deep-sequencing and transcriptome profiling techniques. The objectives are

1) dissecting the genetic factors underlying pollen thermotolerance (PTT) into individual components and
2) identifying cytosin methylation/demethylation patterns that may predispose to PPT.

The project requires experiences in appropriate molecular and bioinformatic techniques.

Where

The group of Peter Winter, GenXPro GmbH, Altenhöferallee 3, 60438 Frankfurt Main, Germany, pwinter@genxpro.de, +49 6995739705.

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